Assembly of the highly repetitive genome of goatgrass

Assembly of the highly repetitive genome of goatgrass

Zimin et al.¬†(lab of Dr. Steven Salzberg) has published a new study describing the assembly of the highly repetitive genome of goatgrass (Aegilops tauschii). The authors introduce the concept of ‘mega-reads’, a hybrid assembly approach that takes advantage of the error-prone but long single-molecule sequencing reads, and the more accurate Illumina sequencing reads. The goatgrass genome is notoriously repetitive and has resisted previous attempts to reveal it’s full sequence. However, it has the preferred¬†property of being almost completely homozygous. The authors use 100x genome coverage of Illumina paired-end 100-250 bp reads, and 10x coverage of PacBio reads. The authors present the impressive N50 contig size of 486,807 nucleotides (i.e., 50% of the bases are present in contigs larger than this size).

One thought on “Assembly of the highly repetitive genome of goatgrass

Comments are closed.

Comments are closed.